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KMID : 1177720090030020011
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Journal of Alternatives to Animal Experiments
2009 Volume.3 No. 2 p.11 ~ p.22
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In vitro Genotoxic evaluation of gold nanoparticles
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Kim Ji-Eun
Lee Seul
Kang Jin-Seok
Yum Young-Na
Kim Dong-Sup
Park Sue-Nie
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Abstract
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Owing to the increasing development of nanotechnology, there is a need to assess how engineered nanomaterials can interact with living cells. It is necessary to assay genotoxic risk of nanomaterials to know the effects on the DNA. We investigated the possible genotoxic potential of polyethylene glycol(PEG) coated gold nanoparticles (AuNP) with four sizes (4, 13, 100 and 200 nm diameter) to investigate the influence of particle size. These AuNP of four sizes at different dose level were exposed to the human bronchial epithelial cell line(BEAS-2B cells) and human lymphoblast cell line(TK6 cells) for 48 hours to see if there is any difference between cell types. Four nm or 100 nm AuNP at a concentration of 250 μg/ml reduced cell viability of TK6 cells more strongly, compared to BEAS-2B cells. However, no significant DNA damage was observed in both BEAS-2B cells and TK6 cells with 4 hours incubations of AuNP in the Comet assay. The results of the Ames assay and the chromosome aberration test using Chinese hamster lung(CHL) cell were also negative. In our
previous study, TNF-α mRNA expression and malonaldehyde level were increased in L5178Y mouse lymphoma cells treated with AuNP. The mechanism of cytotoxicity seems to be oxidative stress. Thus, AuNP are considered non genotoxic in our study condition. However, they could exert oxidative stress mediated cytotoxicity.
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KEYWORD
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Nanomaterial, genotoxicity, gold nanoparticle
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